Topic “probes”
This topics current hotness is 0.4 - That means that it was covered by 0.43 articles per day over the last week.
Since 2007-08-31, this topic was covered by 113 Articles from 53 Feeds.
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2008-06-26
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Old school versus new school in the lab [Respectful Insolence]
[…] element (whichever came first); measuring the copy number of mRNAs in a single cell; identifying microRNAs; doing confocal microscopy on four different fluorescent probes and then confirming it with multiple co-immunoprecipitation to confirm protein interactions of any colocalications observed hard core. Don't worry if you don't understand all that. Those who do understand will know just how hard core (and to some extent old school) I mean, and for the rest of you suffice it to say that I […] read
2008-06-25
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How Facebook servers survive 50,000 new users a day [Clips]
[…] 50,000 new users per day. Heilliger responds: "We're adding a lot of infrastructure and we're adding a lot of servers." That much we understood. But then Farber probes further, asking Heilliger "what's the basic architecture for delivering information at low latency?" And Heilliger answered, losing us for good: Our site is similar to a typical Web three tier architecture. We run a collapsed Web and app tier on the top, which is Apache and PHP, both tremendous open source projects. The […] read
2008-06-21
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Mortgage fraud probes underway: Hundreds indicted
Well, well, Spud fell in the well—Our Gang-Wheezer, 1931 Crooks and Liars, all of them: Federal authorities announced Thursday that more than 400 real estate industry players have been indicted since March — including dozens over the past two days — in nationwide crackdown on mortgage fraud that has contributed to the country’s housing crisis. The FBI [...] read
2008-06-06
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Phylogenetic studies of the genus Cebus (Cebidae-Primates) using chromosome painting and G-banding
Background: Chromosomal painting, using whole chromosome probes from humans and Saguinus oedipus, was used to establish karyotypic divergence among species of the genus Cebus, including C. olivaceus, C. albifrons, C. apella robustus and C. apella paraguayanus. Cytogenetic studies suggested that the species of this genus have conservative karyotypes, with diploid numbers ranging from 2n = 52 to 2n = 54. Results: Banding studies revealed […] read
2008-06-05
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A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids
[…] employed real-time PCR is the high cost of the labeled probe for each target molecule. Results: One real-time PCR technique employing attached universal duplex probes (AUDP) is developed, which is supposed to have the advantage of generating fluorescence by probe hydrolysis and strand displacement over current real-time PCR methods. AUDP is one set of universal duplex probes in which the 5a end of the fluorescent probe (FP) and a complementary quenching probe (QP) lie in close proximity […] read
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GMDD: a database of GMO detection methods
[…] name, exogenous gene, or protein information, etc. In this database, users can obtain the sequences of exogenous integration, which will facilitate PCR primers and probes design. Also the information on endogenous genes, certified reference materials, reference molecules, and the validation status of developed methods is included in this database. Furthermore, registered users can also submit new detection methods and sequences to this database, and the newly submitted information will be […] read
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Probe-specific mixed-model approach to detect copy number differences using multiplex ligation-dependent probe amplification (MLPA)
[…] propose a method for the normalization procedure based on a non-linear mixed-model, as well as a new approach for determining the statistical significance of altered probes based on linear mixed-model.This method establishes a threshold by using different tolerance intervals that accommodates the specific random error variability observed in each test sample. Results: Through simulation studies we have shown that our proposed method outperforms the existing ones based on threshold rule or […] read
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Exon and junction microarrays detect widespread mouse strain- and sex-bias expression differences
[…] representing 9,406 exons. Gene expression was calculated via two different methods, using the 3'-most exon probe ("3' gene expression profiling") and using all probes associated with the gene ("whole-transcript gene expression profiling"), while exon expression was determined using exon probes and flanking junction probes that spanned across the neighboring exons ("exon expression profiling"). Widespread strain and sex influences were detected using a two-way Analysis of Variance […] read
2008-05-27
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Gov probes military surrogates
TV News: Defense Dept. to conduct inquiry -- Under pressure from the House of Representatives, the Defense Dept. will conduct an inquiry into its use of retired military officers as surrogates on network television news. read
2008-05-24
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NEWS: London exhibit probes parallels between architecture and fashion...
The International Herald Tribune have put together a fascinating article about how fashion and architecture meet, we all know to well about Tinker… Read More read